Cloning and characterization of XiR1, a locus responsible for dagger nematode resistance in grape

• DOI: 10.1007/s00122-010-1349-y
• Publication Date: 2010-05-20T08:36:46Z
• Authors: Chin-Feng Hwang; Kenong Xu; Rong Hu; Rita Zhou; Summaira Riaz; M. Andrew Walker
• Keywords: Nepovirus

The dagger nematode, Xiphinema index, feeds aggressively on grape roots and in the process, vectors grapevine fanleaf virus (GFLV) leading to severe viral disease known as degeneration. Resistance X. index GFLV has been key objective of rootstock breeding programs. A previous study found that resistance derived from Vitis arizonica was largely controlled by a major quantitative trait locus, XiR1 (X. 1), located chromosome 19. presented here develops high-resolution genetic physical maps an effort identify gene(s). mapping carried out with 1,375 genotypes three populations D8909-15, resistant selection cross V. rupestris A. de Serres (susceptible) x b42-26 (resistant). evaluated 99 informative recombinants were identified screening two markers flanking locus. map primarily constructed seven DNA developed this study. Physical accomplished bacterial artificial (BAC) libraries vinifera Cabernet Sauvignon b42-26. total 32 BAC clones locus delineated within 115 kb region. Sequence analysis putative nucleotide binding/leucine-rich repeat (NB-LRR) genes. This is first report closely linked gene responsible for ectoparasitic nematode resistance. are being used expedite rootstocks.