Development of a wheat single gene FISH map for analyzing homoeologous relationship and chromosomal rearrangements within the Triticeae

• DOI: 10.1007/s00122-013-2253-z
• Publication Date: 2014-01-09T14:39:27Z
• Authors: Tatiana V. Danilova; Bernd Friebe; Bikram S. Gill
• Keywords: Triticeae; Common wheat; Chromosomal rearrangement; Aegilops

A cytogenetic map of wheat was constructed using FISH with cDNA probes. markers detected homoeology and chromosomal rearrangements wild relatives, an important source genes for improvement. To transfer agronomically from relatives to bread (Triticum aestivum L., 2n = 6 x 42, AABBDD) by induced homoeologous recombination, it is know the relationships species involved. Fluorescence in situ hybridization (FISH) can be used study chromosome structure. The genomes allohexaploid other Triticeae tribe are colinear some extent, i.e., composed homoeoloci at similar positions along chromosomes, genic regions being highly conserved. develop specific homoeologs, we selected more than 60 full-length cDNAs BLAST against mapped expressed sequence tags them as Most probes produced signals on all three chromosomes expected positions. We developed a physical several located each 14 arms. confirmed within were successfully structure species. analysis 1 U-6 U translocation genome Aegilops umbellulata, showed colinearity between Ae. caudata group-1 arm 7S#3 L Thinopyrum intermedium long group-7 chromosomes.