High-resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus

• DOI: 10.1007/s00122-015-2488-y
• Publication Date: 2015-03-09T10:50:45Z
• Authors: Philipp E. Bayer; Pradeep Ruperao; Annaliese S. Mason; Jiri Stiller; Chon-Kit Kenneth ...; Satomi Hayashi; Yan Long; Jinling Meng; Tim Sutton; Paul Visendi; Rajeev K. Varshney; Jacqueline Batley; David Edwards
• Keywords: 0301 basic medicine; Genotype; Genotyping Techniques; Brassica napus; Gene Conversion; Chromosome Mapping; High-Throughput Nucleotide Sequencing; Genetics and Genomics; General Medicine; Polymorphism, Single Nucleotide; 630; Cicer; 03 medical and health sciences; 1311 Genetics; Genetics; 1305 Biotechnology; 1102 Agronomy and Crop Science; Crossing Over, Genetic; Agronomy and Crop Science; Genome, Plant; Biotechnology

We characterise the distribution of crossover and non-crossover recombination in Brassica napus and Cicer arietinum using a low-coverage genotyping by sequencing pipeline SkimGBS. The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene-trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.