Identification and validation of genomic regions influencing kernel zinc and iron in maize

• DOI: 10.1007/s00122-018-3089-3
• Publication Date: 2018-03-24T10:41:36Z
• Authors: Vemuri Hindu; Natalia Palacios-...; Raman Babu; Willy B. Suwarno; Zerka Rashid; Rayalcheruvu Usha; Gajanan R Saykhedkar; Sudha K. Nair
• Keywords: Genome-wide Association Study; Biofortification; Genetic Association

Genome-wide association study (GWAS) on 923 maize lines and validation in bi-parental populations identified significant genomic regions for kernel-Zinc and-Iron maize. Bio-fortification of with elevated Zinc (Zn) Iron (Fe) holds considerable promise alleviating under-nutrition among the world's poor. through molecular breeding could be an economical strategy developing nutritious maize, hence this study, we adopted GWAS to identify markers associated high kernel-Zn Fe subsequently validated marker-trait associations independent populations. For GWAS, evaluated a diverse mapping panel inbred across three environments detected trait using high-density Single nucleotide polymorphism (SNPs) obtained genotyping-by-sequencing. Phenotyping trials showed heritability moderate correlation between concentrations. revealed total 46 SNPs (Zn-20 Fe-26) significantly (P ≤ 5.03 × 10-05) concentrations some these located within previously reported QTL intervals traits. Three double-haploid (DH) were developed from that contrasting micronutrients. The DH phenotyped at two used validating 1 10-03) based single marker analysis. Based analysis, 11 found have effect variance 0.01, R2 ≥ 0.05) least one population. These findings are being pursued program, hold great value functional analysis possible cloning high-value genes traits