OsCOP1 regulates embryo development and flavonoid biosynthesis in rice (Oryza sativa L.)

• DOI: 10.1007/s00122-021-03844-9
• Publication Date: 2021-05-05T15:03:09Z
• Authors: Backki Kim; Rihua Piao; Gileung Lee; Eunbyeol Koh; Yunjoo Lee; Sunmin Woo; Reflinur; Wenzhu Jiang; Endang M. Septini...; Michael J. Thomson; Hee-Jong Koh
• Keywords: Flavonoid Biosynthesis; Genetically modified rice

Novel mutations of OsCOP1 were identified to be responsible for yellowish pericarp and embryo lethal phenotype, which revealed that plays a crucial role in flavonoid biosynthesis embryogenesis rice seed. Successful production viable seeds is major component plant life cycles, seed development complex, highly regulated process affects characteristics such as viability color. In this study, three yellowish-pericarp (yel) mutants, yel-hc, yel-sk, yel-cc, produced from different japonica cultivars (Oryza sativa L). Mutant had pericarps exhibited embryonic lethality, with significantly reduced grain size weight. Morphological aberrations apparent by 5 days after pollination, abnormal increased accumulation observed the yel mutants. Genetic analysis mapping phenotype mutants was controlled single recessive gene, LOC_Os02g53140, an ortholog Arabidopsis thaliana CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1). The yel-cc carried RING finger, coiled-coil, WD40 repeat domains, respectively, OsCOP1. CRISPR/Cas9-targeted mutagenesis used knock out targeting its functional transgenic displayed mutant phenotype. Overexpression homozygous yel-hc background restored color, aberrant endosperm. These results demonstrate associated grains. This study will facilitate better understanding roles involved early seeds.