Allochromatium tepidum, sp. nov., a hot spring species of purple sulfur bacteria

• DOI: 10.1007/s00203-021-02715-7
• Publication Date: 2022-01-04T20:02:47Z
• Authors: Michael T. Madigan; Jill N. Absher; Joseph E. Mayers; Marie Asao; Deborah O. Jung; Kelly S. Bender; Megan L. Kempher; Mackenzie K. Hayward; Sophia A. Sangued...; Abigail C. Brown; Shinichi Takaichi; Ken Kurokawa; Atsushi Toyoda; Hiroshi Mori; Yusuke Tsukatani; Zheng-Yu Wang-Otomo; David M. Ward; W. Matthew Sattley
• Keywords: Hot spring; Strain (injury); Green sulfur bacteria; Purple bacteria; Anoxygenic photosynthesis; Extreme environment

We describe a new species of purple sulfur bacteria (Chromatiaceae, anoxygenic phototrophic bacteria) isolated from a microbial mat in the sulfidic geothermal outflow of a hot spring in Rotorua, New Zealand. This phototroph, designated as strain NZ, grew optimally near 45 °C but did not show an absorption maximum at 915 nm for the light-harvesting-reaction center core complex (LH1-RC) characteristic of other thermophilic purple sulfur bacteria. Strain NZ had a similar carotenoid composition as Thermochromatium tepidum, but unlike Tch. tepidum, grew photoheterotrophically on acetate in the absence of sulfide and metabolized thiosulfate. The genome of strain NZ was significantly larger than that of Tch. tepidum but slightly smaller than that of Allochromatium vinosum. Strain NZ was phylogenetically more closely related to mesophilic purple sulfur bacteria of the genus Allochromatium than to Tch. tepidum. This conclusion was reached from phylogenetic analyses of strain NZ genes encoding 16S rRNA and the photosynthetic functional gene pufM, from phylogenetic analyses of entire genomes, and from a phylogenetic tree constructed from the concatenated sequence of 1090 orthologous proteins. Moreover, average nucleotide identities and digital DNA:DNA hybridizations of the strain NZ genome against those of related species of Chromatiaceae supported the phylogenetic analyses. From this collection of properties, we describe strain NZ here as the first thermophilic species of the genus Allochromatium, Allochromatium tepidum NZT, sp. nov.