Corallococcus silvisoli sp. nov., a novel myxobacterium isolated from subtropical forest soil

• DOI: 10.1007/s00203-021-02725-5
• Publication Date: 2022-01-18T12:02:56Z
• Authors: Xian-Jiao Zhang; Guang-Da Feng; Yang Liu; Yang Zhou; Xiaoqin Deng; Qing Yao; Honghui Zhu
• Keywords: DNA, Bacterial; Soil; 0303 health sciences; 03 medical and health sciences; RNA, Ribosomal, 16S; Fatty Acids; Sequence Analysis, DNA; Forests; Phospholipids; Phylogeny; Soil Microbiology; Bacterial Typing Techniques

An orange-pigmented myxobacterium, designated strain c25j21T, was isolated from subtropical forest soil collected from the Chebaling National Nature Reserve in Guangdong Province, China. Phylogenetic analysis based on the 16S rRNA gene and core genes clearly showed that the novel strain was affiliated within the genus Corallococcus and most closely related to Corallococcus aberystwythensis DSM 108846T (99.3% 16S rRNA gene sequence similarity), while C. exercitus DSM 108849T (99.2%) and C. carmarthensis DSM 108842T (99.0%) were the next most closely related type strains. The draft genome sequence of strain c25j21T was 9.23 Mb in length with a G + C content of 70.7 mol%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain c25j21T and its closely related type strains were 88.1-89.1 and 34.1-36.3%, respectively. The major fatty acids contained iso-C15:0, iso-C17:0, iso-C17:1ω5c and iso-C17:0 2-OH. The predominant respiratory quinone was menaquinone 7. Based on phylogenetic, phenotypic and chemotaxonomic analysis, strain c25j21T represents a novel species of the genus Corallococcus, for which the name Corallococcus silvisoli sp. nov. is proposed. The type strain is c25j21T (= GDMCC 1.1387T = KCTC 62437T).