Quercetin influences intestinal dysbacteriosis and delays alveolar epithelial cell senescence by regulating PTEN/PI3K/AKT signaling in pulmonary fibrosis

• DOI: 10.1007/s00210-023-02913-8
• Publication Date: 2023-12-28T13:02:31Z
• Authors: Wenjuan Wu; Xinhui Wu; Lingxiao Qiu; Ruijie Wan; Xiaoming Zhu; Song Chen; Xinying Yang; Xueya Liu; Jizhen Wu
• Keywords: Senescence

Abstract Pulmonary fibrosis is a chronic and progressive lung disease with high mortality. This study aims to explore the protective mechanism of quercetin against pulmonary regarding cell senescence gut microbiota. Rats were intratracheally injected bleomycin (BLM) establish rat model. RLE-6TN cells stimulated BLM build model alveolar epithelial senescence, RLE-6TN-derived conditional medium (CM) was harvested further culture fibroblasts. Histopathological changes assessed by H&E Masson staining. α-SMA expression immunofluorescence assay. Senescence-associated β-galactosidase (SA-β-gal) staining senescence-associated secretory phenotype (SASP) cytokine assay conducted assess cellular senescence. Gut microbiota analyzed 16S rRNA gene sequencing. The fibrosis-, senescence-, PTEN/PI3K/AKT signaling–related proteins examined western blot. In BLM-induced rats, exerted its effects reducing histological injury collagen deposition, lessening regulating inhibited lessened SASP secretion cells, ameliorated deposition in addition, might exert functional signaling pathway. Moreover, regulated intestinal dysbacteriosis especially boosting abundance Akkermansia . To conclude, our findings provide an in-depth understanding potential behind role fibrosis.