The role of the mobile proton in fucose migration

• DOI: 10.1007/s00216-019-01657-w
• Publication Date: 2019-03-02T01:08:51Z
• Authors: Maike Lettow; Eike Mucha; Christian Manz; Daniel A. Thomas; Mateusz Marianski; Gerard Meijer; Gert von Helden; Kevin Pagel
• Keywords: Proton affinity; Ion-mobility spectrometry

Fucose migration reactions represent a substantial challenge in the analysis of fucosylated glycan structures by mass spectrometry. In addition to well-established observation transposed fucose residues glycan-dissociation product ions, recent experiments show that rearrangement can also occur intact ions. These results suggest low-energy barrier for residue and broaden relevance include other types spectrometry experiments, including ion mobility-mass spectroscopy. this work, we utilize cold-ion infrared spectroscopy provide further insight into scrambling Our mobility proton is prerequisite reaction. For prototypical glycans Lewis x blood group antigen H-2, formation adduct ions or functional groups with variable affinity yields significant differences spectra. changes correlate well promotion inhibition through presence absence mobile proton.