Evaluating the treatment effectiveness of copper-based algaecides on toxic algae Microcystis aeruginosa using single cell-inductively coupled plasma-mass spectrometry

• DOI: 10.1007/s00216-019-01933-9
• Publication Date: 2019-06-14T06:02:31Z
• Authors: Xing Shen; Haiting Zhang; Xiaolong He; Honglan Shi; Chady Stephan; Hua Jiang; Cuihong Wan; Todd Eichholz
• Keywords: Microcystis aeruginosa

Single cell-inductively coupled plasma-mass spectrometry (SC-ICP-MS) is an emerging technology. In this work, we have developed a novel SC-ICP-MS method to quantify metal ions in individual cells of toxic cyanobacterial species, Microcystis aeruginosa (M. aeruginosa), without complicated post-dosing sample preparation, and applied study the treatment effectiveness copper-based algaecides (cupric sulfate EarthTec®) on algae M. aeruginosa. The uses new intrinsic element magnesium determine real transport efficiency cell concentration. viability microcystin-LR release by algaecide were studied flow cytometry ultra-fast liquid chromatography-tandem mass spectrometry, respectively. results showed that was very rapid, highly sensitive (detection limits intracellular copper 65 ag/cell 98 ag/cell, respectively), reproducible (relative standard deviation within 12%). further demonstrated forms cupric EarthTec® successfully diminished populations. higher concentration used treat cells, faster speeds uptake lysis concentrations ranged from 0 200 μg/L algaecide. exhibit obvious heterogeneity uptake. result suggests cumulate followed cellular release. These indicated though could control type harmful algal bloom, remove released toxin treated water would be needed. Graphical abstract.