Validation of MALDI-MS imaging data of selected membrane lipids in murine brain with and without laser postionization by quantitative nano-HPLC-MS using laser microdissection

• DOI: 10.1007/s00216-020-02818-y
• Publication Date: 2020-07-25T22:02:30Z
• Authors: Fabian B. Eiersbrock; Julian M. Orthen; Jens Soltwisch
• Keywords: Laser capture microdissection; MALDI imaging

MALDI mass spectrometry imaging (MALDI-MSI) is a widely used technique to map the spatial distribution of molecules in sectioned tissue. The based on systematic generation and analysis ions from small sample volumes, each representing single pixel investigated surface. Subsequently, spectrometric images for any recorded ion species can be generated by displaying signal intensity at coordinate origin these pixels. Although easily equalized, intensities, however, are not necessarily good measure underlying amount analyte care has taken interpretation MALDI-MSI data. Physical chemical properties that define molecules' adjacencies tissue largely influence local extraction ionization efficiencies, possibly leading strong variations response. Here, we inspect validity distributions murine cerebellum as molar distributions. Based segmentation derived measurements, laser microdissection (LMD) was cut out regions interest with homogenous intensity. concentration six exemplary selected membrane lipids different lipid classes determined using quantitative nano-HPLC-ESI-MS. Comparison concentrations revealed deviations between suggested MSI Determined response factors strongly depend type species. Graphical abstract.