Comparing nanobody and aptamer-based capacitive sensing for detection of interleukin-6 (IL-6) at physiologically relevant levels

• DOI: 10.1007/s00216-023-04973-4
• Publication Date: 2023-10-05T03:21:39Z
• Authors: Raquel Sánchez-Sa...; Rebeca Miranda-Ca...; Noemí de-los-Sant...; María Jesús Lobo-...; Damion K. Corrigan
• Keywords: Aptamer

Abstract A major societal challenge is the development of necessary tools for early diagnosis diseases such as cancer and sepsis. Consequently, there a concerted push to develop low-cost non-invasive methods analysis with high sensitivity selectivity. notable trend highly sensitive that are not only amenable point-of-care ( POC ) testing, but also wearable devices allowing continuous monitoring biomarkers. In this context, test detection promising biomarker, protein Interleukin-6 (IL-6), could represent significant advance in clinical management cancer, chemotherapy response, or prompt This work reports capacitive electrochemical impedance spectroscopy sensing platform tailored towards treatment human serum. The specific recognition IL-6 was achieved employing gold surfaces modified an anti-IL6 nanobody (anti-IL-6 VHH) aptamer. first system, anti-IL-6 VHH covalently attached surface using binary self-assembled-monolayer (SAM) 6-mercapto-1-hexanol (MCH) 11-mercaptoundecanoic acid. second aptamer chemisorbed onto mixed SAM layer MCH. analytical performance each label-free sensor evaluated buffer 10% serum samples then compared. results were generated low-cost, thin film eight-channel array produced on flexible substrate providing useful information future design biomarker platforms.