Hypolipidemic mechanism of gypenosides via inhibition of pancreatic lipase and reduction in cholesterol micellar solubility

• DOI: 10.1007/s00217-015-2540-9
• Publication Date: 2015-09-05T15:56:22Z
• Authors: Jianhui Su; Hongxin Wang; Chaoyang Ma; Chengxiang Liu; MdRamim Tanver Ra...; Chuanzhong Gao; Rongjing Nie
• Keywords: 0301 basic medicine; 03 medical and health sciences

Effect of gypenosides on porcine pancreatic lipase (PL) activity was determined in vitro. Gypenosides inhibited the PL activity in a dose-dependent manner at the concentrations of 0–1.0 mg/ml. The Lineweaver–Burk plots for gypenosides indicated that the inhibition on PL by gypenosides was noncompetitive. The conformation of PL was investigated by means of circular dichroism and fluorescence. The result revealed the decrease in α-helix contents, increase in β-sheet and the decrease in fluorescence intensity after gypenosides treatment. The incorporation of cholesterol into micelles was inhibited by gypenosides. A complex was observed by transmission electron microscopy, indicating interaction between cholesterol and gypenosides which led to the decrease in cholesterol micelle solubility. The result revealed the fact that gypenosides played a role in inhibiting PL activity and reducing intestinal cholesterol absorption via inhibition of micelle formation.