Impact of Dietary Aromatic Amino Acids on Osteoclastic Activity

• DOI: 10.1007/s00223-014-9878-z
• Publication Date: 2014-07-07T07:02:10Z
• Authors: Mona El Refaey; Qing Zhong; Ke-Hong Ding; Xing-ming Shi; Jianrui Xu; Wendy B. Bollag; William D. Hill; Norman Chutkan; Richard Robbins; Hugh Nadeau; Maribeth Johnson; Mark W. Hamrick; Carlos M. Isales
• Keywords: Cathepsin K; Calcitonin receptor

We had shown that aromatic amino acid (phenylalanine, tyrosine, and tryptophan) supplementation prevented bone loss in an aging C57BL/6 mice model. In vivo results from the markers of breakdown suggested inhibition osteoclastic activity or differentiation. To assess differentiation, we examined effects acids on early /structural as vitronectin receptor, calcitonin carbonic anhydrase II well as, late/functional differentiation markers; cathepsin K matrix metalloproteinase 9 (MMP-9). Our data demonstrate down-regulated late measured by real time PCR. also suggest a link between receptor secreted both showed consistent to treatment. However, non-attachment related proteins, II, demonstrated less response are with down-regulating suppressing remodeling gene expression thus contributing initially net increase mass seen vivo.