Contribution of Cell Walls, Nonprotein Thiols, and Organic Acids to Cadmium Resistance in Two Cabbage Varieties

• DOI: 10.1007/s00244-012-9824-x
• Publication Date: 2012-10-30T11:06:50Z
• Authors: Jianyun Sun; Jin Cui; Chunling Luo; Lu Gao; Yahua Chen; Zhenguo Shen
• Keywords: 0301 basic medicine; 03 medical and health sciences; Cell Wall; Phytochelatins; Soil Pollutants; Brassica; Sulfhydryl Compounds; Adaptation, Physiological; Cadmium

To study possible cadmium (Cd) resistance mechanisms in cabbage (Brassica oleracea L.), several parameters of metal uptake, distribution, and complexation were compared between two varieties Chunfeng [CF (Cd-tolerant)] and Lvfeng [LF (Cd-sensitive)]. Results showed that CF contained significantly lower Cd concentrations in leaves and higher Cd concentrations in roots than LF. Approximately 70 to 74 % and 66 to 68 % of Cd taken up by LF and CF, respectively, was transported to shoots. More Cd was bound to the cell walls of leaves, stems, and roots in CF than in LF. The higher capacity of CF to limit Cd uptake into shoots could be explained by immobilization of Cd in root cell walls. Compared with control groups, Cd treatment also significantly increased concentrations of nonprotein thiols, phytochelatins (PCs), and citric acid in the leaves and roots of the two varieties; the increases were more pronounced in CF than in LF. Taken together, the results suggest that the greater Cd resistance in CF than in LF may be attributable to the greater capacity of CF to limit Cd uptake into shoots and complex Cd in cell walls and metal binding ligands, such as PCs and citric acid. However, the contributions of PCs and citric acid to Cd detoxification might be smaller than those in cell walls.