16S rDNA Pyrosequencing Analysis of Bacterial Community in Heavy Metals Polluted Soils

• DOI: 10.1007/s00248-013-0344-7
• Publication Date: 2014-01-08T10:53:37Z
• Authors: Marcin Gołębiewski; Edyta Deja-Sikora; Marcin Cichosz; Andrzej Tretyn; Borys Wróbel
• Keywords: Pyrosequencing; Soil microbiology

Soil contamination with heavy metals is a widespread problem, especially prominent on grounds lying in the vicinity of mines, smelters, and other industrial facilities. Many such areas are located Southern Poland; they polluted mainly Pb, Zn, Cd, or Cu, locally also Cr. As for now, little known about most bacterial species thriving soils even less core community--a set taxa common to soils. Therefore, we wanted answer question if could be found samples differing physicochemically phytosociologically. To question, analyzed communities three soil contaminated Pb Zn two Cr lower levels Zn. The were assessed 16S rRNA gene fragments pyrosequencing. It was that differed significantly decreased both diversity richness at family levels, while plant did not correlate diversity. In spite differences between samples, shared many operational taxonomic units (OTUs) it possible delineate microbiome our sample set. OTUs comprised members as Sphingomonas, Candidatus Solibacter, Flexibacter showing particular genera might among sites ~40 km distant.