Aspergillus niger uses the peroxisomal CoA-dependent β-oxidative genes to degrade the hydroxycinnamic acids caffeic acid, ferulic acid, and p-coumaric acid

• DOI: 10.1007/s00253-021-11311-0
• Publication Date: 2021-05-05T08:03:05Z
• Authors: R. J. M. Lubbers; A. Dilokpimol; J. Visser; R. P. de Vries
• Keywords: Hydroxycinnamic acid; Aspergillus niger; Coumaric acid

Abstract Aromatic compounds are important molecules which widely applied in many industries and mainly produced from nonrenewable sources. Renewable sources such as plant biomass interesting alternatives for the production of aromatic compounds. Ferulic acid p- coumaric acid, a precursor vanillin p -vinyl phenol, respectively, can be released by fungus Aspergillus niger . The degradation hydroxycinnamic acids caffeic ferulic -coumaric has been observed fungi. In A. , multiple metabolic pathways were suggested acids. However, no genes identified these pathways. this study, several pathway using whole-genome transcriptomic data grown on different involved CoA-dependent β-oxidative This is well known fatty acids, but not plants, it shown that degraded through pathway. We encoding hydroxycinnamate-CoA synthase ( hcsA ), multifunctional β-oxidation hydratase/dehydrogenase foxA 3-ketoacyl CoA thiolase katA four thioesterases theA-D ) highly induced all three tested Deletion mutants revealed indeed addition, theB also HcsA, FoxA, KatA contained peroxisomal targeting signal therefore predicted to localized peroxisomes. Key points • Metabolism was investigated Using transcriptome data, identified. Both hydroxycinnamate metabolism.