Basidiomycota strains as whole-cell biocatalysts for the synthesis of high-value natural benzaldehydes

• DOI: 10.1007/s00253-023-12872-y
• Publication Date: 2024-01-11T12:01:47Z
• Authors: Stefano Serra; Stefano Marzorati; Ewa Szczepańska; Tomasz Strzała; Filip Boratyński
• Keywords: Benzoic acid; Biotransformation

Abstract Substituted benzaldehydes are the most commonly used natural-occurring flavours in world. The consumer’s preference for ‘natural or organic’ aromas has increased request possessing ‘natural’ status. resulting shortage of aromatic aldehydes extractive origin, such as vanillin, veratraldehyde and piperonal, can be offset by developing a new biotechnological synthesis method. Here, we report study on microbiological reduction five natural benzoic acid derivatives, namely p -anisic, vanillic, veratric, piperonylic eudesmic acids, to produce corresponding fragrant aldehydes. We found that different Basidiomycota strains efficiently perform this transformation, with good chemical selectivity tolerance toxicity substrates products. Besides confirming carboxylic reductase activity already studied fungi Pycnoporus cinnabarinus , discovered other species Pleurotus eryngii sapidus Laetiporus sulphureus well non-ligninolytic Lepista nuda valuable microorganisms anisaldehyde, veratraldehyde, piperonal 3,4,5-trimethoxybenzaldehyde from acids. According our findings, propose reliable process preparation above-mentioned aldehydes, form. Key points • Fragrant were obtained biotransformation. reduced substituted Anisaldehyde, prepared Graphical