A type III polyketide synthase cluster in the phylum Planctomycetota is involved in alkylresorcinol biosynthesis

• DOI: 10.1007/s00253-024-13065-x
• Publication Date: 2024-02-26T10:02:44Z
• Authors: Lars Milke; Moses Kabuu; Renè Zschoche; Jochem Gätgens; Karin Krumbach; Kim-Loreen Carlstedt; Carmen E. Wurzbacher; Sven Balluff; Christine Beemelm...; Christian Jogler; Jan Marienhagen; Nicolai Kallscheuer
• Keywords: Polyketide synthase; Corynebacterium glutamicum; Gene cluster; Heterologous expression; Streptomyces albus

Abstract Members of the bacterial phylum Planctomycetota have recently emerged as promising and for most part untapped sources novel bioactive compounds. The characterization more than 100 species in last decade stimulated recent bioprospection studies that start to unveil chemical repertoire phylum. In this study, we performed systematic bioinformatic analyses based on genomes all 131 described members current focusing identification type III polyketide synthase (PKS) genes. Type PKSs are versatile enzymes involved biosynthesis a wide array structurally diverse natural products with potent biological activities. We identified 96 putative PKS genes which 58 encoded an operon encoding oxidoreductase methyltransferase. Sequence similarities protein level genetic organization point towards functional link related hierridins discovered picocyanobacteria. heterologous expression planctomycetal from strains belonging different families engineered Corynebacterium glutamicum strain led pentadecyl- heptadecylresorcinols. Phenotypic assays producer constructed gene deletion mutant suggest function compounds differs confirmed other alkylresorcinol producers. Key points • Planctomycetal synthases synthesize long-chain alkylresorcinols. Phylogenetic ecological picocyanobacterial hierridins. Engineered C. is suitable planctomycete-derived