Updated component analysis method for naturally occurring sophorolipids from Starmerella bombicola

• DOI: 10.1007/s00253-024-13138-x
• Publication Date: 2024-04-12T08:01:55Z
• Authors: Yosuke Kobayashi; Qiushi Li; Kazunori Ushimaru; Makoto Hirota; Tomotake Morita; Tokuma Fukuoka
• Keywords: Glyceride

Abstract Sophorolipids (SLs) are promising glycolipid biosurfactants as they easily produced and functional. SLs from microorganisms comprised of mixtures multiple derivatives that have different structures properties, including well-known acidic lactonic SL (ASLs LSLs, respectively). In this study, we established a method for analyzing all in the products Starmerella bombicola , typical SL-producing yeast. Detailed component analyses S. were carried out using reversed-phase high-performance liquid chromatography mass spectrometry. Methanol was used eluent it is good solvent derivatives. With approach, possible to not only quantify ratio main components ASL, LSL, glycerides but also confirm trace such mono-glyceride bola-form (sophorose at both ends); notably, first time these been isolated identified successfully naturally occurring SLs. addition, our results revealed novel derivative which fatty acid bonded series had reported previously. Using present analysis method, track compositional changes during culture. Our showed LSL ASL initially accumulate middle stage fermentation process. Key points • An easy detailed sophorolipids introduced. Multiple known A hydrophobic characterized. Graphical