Enhancement of exogenous protein stability in AcMNPV by overexpressing lef5 gene during passaging

• DOI: 10.1007/s00253-025-13451-z
• Publication Date: 2025-03-24T22:44:47Z
• Authors: Jie Pei; Ruilun Liu; Wei Li; Shasha Qian; Jie Yang; Shengli Meng; Shuo Shen; Jing Guo
• Keywords: Autographa californica; Sf9; Virus-like particle

The baculovirus insect cell expression system is pivotal for exogenous protein expression. However, serial passages of in cells often result defective virus generation and a rapid decline expression, limiting its wider application. Previous studies have shown that the late factor 5 (lef5) from other baculoviruses can enhance stability genes Autographa californica multiple nucleopolyhedrovirus (AcMNPV). In this study, we engineered diverse recombinant strains AcMNPV to express enhanced green fluorescent (eGFP) enterovirus 71 virus-like particles (EV71-VLPs), co-expressed with P1 3CD proteins EV71. We investigated influence lef5 overexpression, regulated by various promoters, on their expressions. Notably, overexpression significantly improved eGFP EV71-P1 gene expressions during Sf9 cells. Specifically, driven op166 promoter was more beneficial enhancing complex proteins, such as EV71-VLPs. This study underscores importance maintaining systems, particularly producing VLPs enterovirus. • stabilizes system. Promoter choice affects lef5-mediated stability. crucial like