An immunosuppressive macrophage profile attenuates the prognostic impact of CD20-positive B cells in human soft tissue sarcoma

• DOI: 10.1007/s00262-019-02322-y
• Publication Date: 2019-03-16T06:02:58Z
• Authors: Panagiotis Tsagozis; Martin Augsten; Yifan Zhang; Tian Li; Asle Hesla; Jonas Bergh; Felix Haglund; Nicholas P. Tobin; Monika Ehnman
• Keywords: CD163

Immune cells can regulate disease progression and response to treatment in multiple tumor types, but their activities human soft tissue sarcoma are poorly characterized.Marker-defined immune cell subsets were characterized from a microenvironmental perspective two independent cohorts of by multiplex IHC, quantitative PCR and/or bioinformatics.B profiling revealed prognostic role for CD20 protein (cohort 1, 33 patients) MS4A1 gene expression 2, 265 patients). Multiplex IHC correlation analysis supported antigen presentation, differentiation T activation. The expressing B was only observed an IL10low, PTGS2low or CD163low microenvironment according the transcriptomic data. IL10 levels consistently correlated with M2-like macrophage marker CD163, which also defined majority macrophages. A polarization these toward pro-tumoral phenotype further lack between CD163 M1 markers like NOS2, as well low abundance CD80 positive tissue.Analysis CD20/MS4A1 merits attention promising candidate tool survival, not patients pronounced immunosuppressive microenvironment. Macrophages ubiquitous polarized protumoral phenotype. This provides rationale studies on function immunotherapy targeting M2-polarized