CD30+OX40+ Treg is associated with improved overall survival in colorectal cancer

• DOI: 10.1007/s00262-021-02859-x
• Publication Date: 2021-02-02T03:04:54Z
• Authors: Jian Hang Lam; Michelle Hong; Si-Lin Koo; Clarinda Wei Ling...; Kah Ling Lim; Felicia Wee; Wei Keat Wan; Wei Qiang Leow; Joo Guan Yeo; Iain Bee Huat Tan; Joe Yeong; Tony Kiat Hon Lim; Tong Seng Lim
• Keywords: 0303 health sciences; Ki-1 Antigen; Receptors, OX40; T-Lymphocytes, Regulatory; 3. Good health; 03 medical and health sciences; Biomarkers, Tumor; Humans; Leukocyte Common Antigens; Original Article; Prospective Studies; Receptors, Cytokine; Colorectal Neoplasms; Cells, Cultured; Retrospective Studies

Regulatory T cells (Tregs) are often enriched in tumors, where their immunosuppressive function has a key role tumor persistence and progression. In colorectal cancer (CRC), however, Tregs frequently associated with an improved clinical outcome. Tumor-infiltrating have been shown to exhibit distinct signature comprising the co-stimulatory molecules (OX40, 4-1BB), cytokine receptors (IL1R2, IL21R, CCR8, CD30), co-inhibitory (PD-L1, TIGIT). Here, we showed by flow cytometry that circulating CD45RO+ from patients CRC (n = 25) elevated CD30 OX40 expression compared healthy subjects 14). We identified co-expression of on using single-cell images captured DEPArray™ system. The frequency CD30+OX40+CD45RO+ was significantly higher than (P < 0.001). Importantly, receiver operating characteristic analysis confirmed this CD30+OX40+ Treg subset could strongly discriminate between highest accuracy 92.3%, AUC 0.92, sensitivity 88%, specificity 100%, positive predictive value negative 82.35%, trade-off 3.44%, other subsets. Consistently, multiplex-IHC/IF tumor-infiltrating revealed significant association high densities overall survival; no such found for These data suggest potential as diagnostic or prognostic biomarker CRC.