Predictive role of minimal residual disease and log clearance in acute myeloid leukemia: a comparison between multiparameter flow cytometry and Wilm’s tumor 1 levels

• DOI: 10.1007/s00277-014-2029-9
• Publication Date: 2014-02-19T05:33:42Z
• Authors: Giovanni Rossi; Maria Marta Miner...; Lorella Melillo; Francesco di Nardo; Chiara de Waure; Potito Rosario Sc...; Gianni Perla; Daniela Valente; Nicola Sinisi; Nicola Cascavilla
• Keywords: Adult; Male; Genes, Wilms Tumor; Neoplasm, Residual; Middle Aged; Flow Cytometry; Wilms Tumor; Disease-Free Survival; Kidney Neoplasms; Acute myeloid leukemia; Log clearance; Minimal residual disease; Multiparameter flow cytometry; WT1-RNA; Hematology; Leukemia, Myeloid, Acute; Young Adult; 03 medical and health sciences; 0302 clinical medicine; Predictive Value of Tests; Humans; Female

In acute myeloid leukemia (AML), the detection of minimal residual disease (MRD) as well as the degree of log clearance similarly identifies patients with poor prognosis. No comparison was provided between the two approaches in order to identify the best one to monitor follow-up patients. In this study, MRD and clearance were assessed by both multiparameter flow cytometry (MFC) and WT1 expression at different time points on 45 AML patients achieving complete remission. Our results by WT1 expression showed that log clearance lower than 1.96 after induction predicted the recurrence better than MRD higher than 77.0 copies WT1/10(4) ABL. Conversely, on MFC, MRD higher than 0.2 % after consolidation was more predictive than log clearance below 2.64. At univariate and multivariate analysis, positive MRD values and log clearance below the optimal cutoffs were associated with a shorter disease-free survival (DFS). At the univariate analysis, positive MRD values were also associated with overall survival (OS). Therefore, post-induction log clearance by WT1 and post-consolidation MRD by MFC represented the most informative approaches to identify the relapse. At the optimal timing of assessment, positive MRD and log-clearance values lower than calculated thresholds similarly predicted an adverse prognosis in AML.