Pseudomonas extremaustralis sp. nov., a Poly(3-hydroxybutyrate) Producer Isolated from an Antarctic Environment

• DOI: 10.1007/s00284-009-9469-9
• Publication Date: 2009-08-17T17:21:09Z
• Authors: Nancy I. López; M. Julia Pettinari; Erko Stackebrandt; Paula M. Tribelli; Markus Põtter; Alexander Steinbü...; Beatriz S. Méndez
• Keywords: DNA, Bacterial; 0303 health sciences; 03 medical and health sciences; Polyesters; Pseudomonas; RNA, Ribosomal, 16S; Molecular Sequence Data; Antarctic Regions; Hydroxybutyrates; DNA, Ribosomal; Phylogeny

A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3(T)) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3(T) was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogenetic affiliation. Strain 14-3(T) is closely related to Pseudomonas veronii DSM 11331(T), sharing 99.7% sequence similarity. DNA-DNA hybridization experiments between the two strains showed only moderate reassociation similarity (35.1%). Tests for arginine dihydrolase and nitrate reduction were positive, while those for denitrification, indol production, glucose acidification, urease, ss-galactosidase, esculin, caseine and gelatin hydrolysis were negative. Growth of this bacterium occurred in a range from 4 to 37 degrees C but not at 42 degrees C. It accumulated poly(3-hydroxybutyrate) when grown on sodium octanoate medium. Strain 14-3(T) therefore represents the type strain of a new species, for which the name Pseudomonas extremaustralis sp. nov. is proposed. The type strain 14-3(T) has been deposited as DSM 17835(T) and as CIP 109839(T).