Hormonal requirements for effective induction of microspore embryogenesis in triticale (× Triticosecale Wittm.) anther cultures

• DOI: 10.1007/s00299-014-1686-4
• Publication Date: 2014-09-26T11:42:22Z
• Authors: Iwona Żur; Ewa Dubas; Monika Krzewska; Piotr Waligórski; Michał Dziurka; Franciszek Janowiak
• Keywords: Zeatin; Triticale; Kinetin; Plant Physiology

Effective microspore embryogenesis in triticale is determined by a specific hormonal homeostasis: low value of IAA/cytokinins, IAA/ABA and cytokinins/ABA ratios as well proper endogenous/exogenous auxin balance, which favours androgenic structure formation green plant regeneration ability. The concentration growth regulators (PGRs): auxins (Auxs), cytokinins (CKs) abscisic acid (ABA) was measured anthers eight DH lines (× Triticosecale Wittm.), associated with (ME) responsiveness. analysis conducted on excised from control tillers at the phase optimal for ME induction then after ME-initiating treatment (21 days 4 °C). In control, IAA predominated among Auxs (11-39 nmol g(-1) DW), IBA constituting only 1 % total content. prevailing isoforms CKs were cis isomers zeatin (121-424 pmol DW) ryboside (cZR, 146-432 DW). Surprisingly, relatively high level (10-64 kinetin (KIN) detected. Cold significantly changed levels all analysed PGRs. 'responsive' contained higher concentrations IBA, trans zeatin, cZR ABA, lower amount KIN comparison 'recalcitrant' genotypes. However, effects exogenous p-chlorophenoxyisobutyric (PCIB) 2,3,5-triiodobenzoic treatments suggest that none studied PGRs acts alone acquisition embryogenic competency, seems to be an effect concerted crosstalk. initiation required certain threshold ABA. A crucial prerequisite effectiveness CKs/ABA ratio. balance between endogenous supplied culture media also essential.