Printing biological solutions through laser-induced forward transfer

• DOI: 10.1007/s00339-008-4741-6
• Publication Date: 2008-06-26T09:19:23Z
• Authors: M. Duocastella; J. M. Fernández-P...; J. Domínguez; P. Serra; J. L. Morenza
• Keywords: Biomolecule; Lift (data mining); Energy density

Laser-induced forward transfer (LIFT) is a direct-writing technique adequate for the high-resolution printing of a wide range of materials, including biological molecules. In this article, the preparation through LIFT of microarrays of droplets from a solution containing rabbit antibody immunoglobulin G (IgG) is presented. The microarrays were prepared at different laser pulse energy conditions, obtaining microdroplets with a circular and well-defined contour. The transfer process has a double threshold: a minimum energy density required to generate an impulsion on the liquid film, and a minimum pulse energy, which corresponds to the onset for material ejection. In addition, it was demonstrated that the transfer process can be correctly described through a simple model which relates the energy density threshold with the amount of released material. Finally, a fluorescence assay was carried out in which the preservation of the activity of the transferred biomolecules was demonstrated.