Salicylic Acid Confers Salt Tolerance in Giant Juncao Through Modulation of Redox Homeostasis, Ionic Flux, and Bioactive Compounds: An Ionomics and Metabolomic Perspective of Induced Tolerance Responses

• DOI: 10.1007/s00344-022-10581-w
• Publication Date: 2022-01-31T05:03:41Z
• Authors: Kashif Hayat; Yuanfei Zhou; Saiqa Menhas; Sikandar Hayat; Tariq Aftab; Jochen Bundschuh; Pei Zhou
• Keywords: 580; 0301 basic medicine; 03 medical and health sciences; Antioxidative defense systems; Saline agriculture; Bioactive compounds; Non-conventional crops; Salinity tolerance

We investigated the stimulatory and/or inhibitory role of exogenous SA in alleviating the salt stress (250, 500 mM NaCl) in Pennisetum giganteum (Giant Juncao) through coordinated induction of redox homeostasis, ionic flux, and bioactive compounds. Salt stress radically impaired root and shoot (growth, fresh, and dry biomass as well as tolerance indices), leaf relative water content, and leaf chlorophyll a/b ratio of Juncao due to higher Na+ and Cl− accumulation followed by H2O2 generation, lipid peroxidation (MDA contents), and electrolyte leakage. However, the innate defense response of Juncao counteracted salt-induced damages by osmolytes accumulation combined with orchestrating antioxidants and ionic homeostasis mechanisms. Furthermore, the application of SA had an incremental impact on the development and productivity of high-salinity-exposed Juncao plants by increasing root length, plant biomass, tolerance indices, chlorophyll a/b ratio, and protein contents. Furthermore, SA treatment considerably decreased Na+ and Cl− toxicity by orchestrating antioxidant enzymes, ion transport, and secondary metabolism. Notably, the application of SA substantially mitigated the adverse effects of high salinity concentration (500 mM NaCl), owing to the simultaneous upregulation in enzymatic and non-enzymatic antioxidants, nutrient ion flux, alongside chlorogenic acid production. Thus, we concluded that SA enhanced the tolerance capability of Juncao plants in a NaCl concentration-dependent manner. The findings of this study will enable environmentalists and pharmacologists to gain dual farm-level benefits, including animal therapeutics and restoration of salinized soils for arable purposes.