Mono- and multi-nucleated ventricular cardiomyocytes constitute a transcriptionally homogenous cell population

• DOI: 10.1007/s00395-019-0744-z
• Publication Date: 2019-08-09T09:03:01Z
• Authors: Michail Yekelchyk; Stefan Guenther; Jens Preussner; Thomas Braun
• Keywords: CD31

Abstract Individual adult ventricular cardiomyocytes are either mono- or multi-nucleated and undergo morphological changes during cardiac hypertrophy. However, corresponding transcriptional signatures, reflecting potentially different functions the ability for cell-cycle entry, not known. The aim of this study was to determine profile by single-cell RNA-sequencing (scRNA-seq) investigate heterogeneity among under baseline conditions in pressure-induced We developed an array-based approach scRNA-seq rod-shaped from both healthy hypertrophic hearts. Single-cell transcriptomes were highly similar, although a certain degree variation noted across populations. Non-image-based quality control allowing inclusion damaged generated artificial cell clusters demonstrating need strict exclusion criteria. In contrast, isolated heart after transverse aortic constriction showed heterogeneous characteristic hypoxia-induced responses. Immunofluorescence analysis revealed inverse correlation between HIF1α + cells CD31-stained vessels, suggesting that imbalanced vascular growth hypertrophied induces cellular heterogeneity. Our demonstrates individual express nearly identical sets genes. Homogeneity lost induction hypertrophy due differential HIF1α-dependent responses most likely caused none-homogenous vessel growth.