Analysis of gene expression dynamics revealed delayed and abnormal epidermal repair process in aged compared to young skin

• DOI: 10.1007/s00403-015-1551-5
• Publication Date: 2015-03-05T01:49:00Z
• Authors: Peggy Sextius; Claire Marionnet; Charlotte Tacheau; François-Xavier Bon; Philippe Bastien; Alain Mauviel; Bruno A. Bernard; Françoise Bernerd; Louis Dubertret
• Keywords: Transepidermal water loss; Epidermis (zoology); Barrier function; Skin Aging

With aging, epidermal homeostasis and barrier function are disrupted. In a previous study, we analyzed the transcriptomic response of young skin epidermis after stratum corneum removal, obtained global kinetic view molecular processes involved in recovery. present same analysis was performed aged order to better understand defects which occur with aging. Thirty healthy male volunteers (67 ± 4 years old) were involved. Tape-strippings carried out on inner face one forearm, other unstripped forearm serving as control. At 2, 6, 18, 30 72 h stripping, TEWL measurements taken, samples collected. Total RNA extracted using DermArray(®) cDNA microarrays. The results highlighted that recovery overall kinetics gene expression delayed following stripping skin. Indeed, showed group appeared be dramatically significant during kinetics, while there no evolution until h. Moreover, revealed number modulated genes tape increased time reached peak at 6 skin, it showing cellular activity linked repair process may engaged earlier than epidermis. A total 370 group. group, 382 modulated, whose 184 also Only eight both groups significantly differently modulated. characterization these into 15 functional families helped draw scenario for aging affecting capacity.