Persistence of DNA threads in human anaphase cells suggests late completion of sister chromatid decatenation

• DOI: 10.1007/s00412-007-0131-7
• Publication Date: 2007-11-07T06:45:57Z
• Authors: Lily Hui-Ching Wang; Thomas Schwarzbraun; Michael R. Speicher; Erich A. Nigg
• Keywords: Spindle checkpoint; Telophase

PICH (Plk1-interacting checkpoint helicase) was recently identified as an essential component of the spindle assembly and shown to localize kinetochores, inner centromeres, thin threads connecting separating chromosomes even during anaphase. In this paper, we have used immuno-fiber fluorescence in situ hybridization chromatin-immunoprecipitation demonstrate that associates with centromeric chromatin Furthermore, by careful analysis PICH-positive anaphase through FISH well bromo-deoxyurdine CREST labeling, strengthen evidence these comprise mainly alphoid centromere deoxyribonucleic acid. Finally, timing addition ICRF-193 (a specific inhibitor topoisomerase-II alpha) cells synchronized anaphase, topoisomerase activity is required specifically resolve (as opposed being prevent formation such earlier cell cycle stages). These data indicate centromeres most evolve from stretch response tension. Moreover, they show for resolution threads, implying complete separation sister chromatids occurs later than previously assumed.