Transepithelial electrical resistance (TEER): a functional parameter to monitor the quality of oviduct epithelial cells cultured on filter supports

• DOI: 10.1007/s00418-015-1351-1
• Publication Date: 2015-07-30T02:32:15Z
• Authors: Shuai Chen; Ralf Einspanier; Jennifer Schoen
• Keywords: Oviduct

Cultivation of oviduct epithelial cells on porous filters fosters in vivo-like morphology and functionality. However, due to the optical properties filter materials cells’ columnar shape, cell quality is hard assess via light microscopy. In this study, we aim evaluate transepithelial electrical resistance (TEER) measurement as a prognostic indicator for cultivation porcine (POEC). POEC were maintained four different types media 3 6 w achieve diverse culture qualities, TEER was measured before processing samples histology. Culture scored using morphological criteria (presence cilia, confluence polarity). We furthermore analyzed correlation between cellular height (as measure apical–basal polarization) fully differentiated routine cultures (biological variation) with altered hormonal stimulation. Fully possessed moderate 500 1100 Ω*cm2. Only 5 % which exhibited values defined range had poor quality. Sub-differentiated showed either very low or excessively high TEER. unveiled highly significant (P < 0.0001) negative linear well-differentiated (both hormone stimulated group). This may point toward interaction tight junction assembly polarization. conclusion, straightforward could be routinely used monitor differentiation status vitro.