Potential functions of embryonic cardiac macrophages in angiogenesis, lymphangiogenesis and extracellular matrix remodeling

• DOI: 10.1007/s00418-020-01934-1
• Publication Date: 2021-01-20T13:03:43Z
• Authors: Grzegorz Gula; Sławomir Rumiński; Justyna Niderla-B...; Agnieszka Jasińska; Ewelina Kiernozek; Ewa Jankowska-Ste...; Aleksandra Flaht-...; Anna Ratajska
• Keywords: Lymphangiogenesis; Podoplanin; Arteriogenesis

Abstract The role of cardiac tissue macrophages (cTMs) during pre- and postnatal developmental stages remains in many aspects unknown. We aimed to characterize cTM populations their potential functions based on surface markers. Our situ studies immunostained specimens murine fetuses (from E11to E17) revealed that a significant number embryonic cTMs (phenotyped by CD45, CD68, CD64, F4/80, CD11b, CD206, Lyve-1) resided mostly the subepicardial space, not entire myocardial wall, as observed adult individuals. accompanied newly developed blood lymphatic vessels adhering vessel walls cellular processes. A subpopulation CD68-positive cells was found form accumulations areas massive apoptosis outflow tract remodeling shortening. Flow cytometry analysis at E14 E17 defined three subpopulations:CD64 low , CD64 high CD206-and CD206+. levels mRNA expression for genes related regulation angiogenesis ( VEGFa VEGFb VEGFc bFGF ), lymphangiogenesis ) extracellular matrix (ECM) MMP13 Arg1 Ym1/Chil3 Retlna/FIZZ1 differed among selected and/or stages. results demonstrate diversity tissue-specific locations, suggesting various roles regulating angiogenesis, ECM remodeling.