Skeletal muscle signaling response to sprint exercise in men and women

• DOI: 10.1007/s00421-011-2164-0
• Publication Date: 2011-09-17T05:01:21Z
• Authors: Teresa Fuentes; Borja Guerra; Jesús G. Ponce-Go...; David Morales-Alamo; Amelia Guadalupe-...; Hugo Olmedillas; Lorena Rodríguez-...; David Feijoo; Pedro De Pablos-V...; Leandro Fernández...; Alfredo Santana; Jose A. L. Calbet
• Keywords: Adult; Male; STAT3 Transcription Factor; 0301 basic medicine; 241106 Fisiología del ejercicio; Gene-Expression; AMP-Activated Protein Kinases; p38 Mitogen-Activated Protein Kinases; Running; Metabolic-Response; Young Adult; 03 medical and health sciences; Messenger-Rna; Humans; Phosphorylation; Muscle, Skeletal; Sex Characteristics; Resistance Exercise; Glucose-Uptake; Repeated Bouts; Leptin Receptors; Gender-Differences; Exercise Test; Female; Interval Exercise; Ampk Activation; Acetyl-CoA Carboxylase; Signal Transduction

To determine if there is a sex dimorphism in the skeletal muscle signaling response to sprint exercise, 17 men and ten women performed a 30-s Wingate test. Muscle biopsies were taken before, immediately after the exercise and at 30 and 120 min during the recovery period. Thr(172)-AMPKα, Ser(221)-ACCβ, Thy(705)-STAT3, Thr(202)/Thy(204)-ERK1/2 and Thr(180)/Thy(182)-p38MAPK phosphorylation responses to sprint exercise were not statistically different between men and women. AMPKα phosphorylation was enhanced fourfold 30 min after the sprint exercise in males and females (P < 0.01). ACCβ phosphorylation was enhanced by about threefold just after the sprint test exercise and 30 min into the recovery period in males and females (P < 0.01). STAT3 phosphorylation was increased 2 h after the Wingate test compared to the value observed right after the end of the exercise (P < 0.05), and 30 min after the Wingate test there was a 2.5-fold increase in ERK1/2 phosphorylation, compared to both the pre-exercise and to the value observed right after the Wingate test (both, P < 0.05). In conclusion, the skeletal muscle signaling response to a single bout of sprint exercise mediated by AMPK, ACC, STAT3, ERK and p38MAPK is not statistically different between men and women. Marked increases in AMPKα, ACCβ, STAT3 and ERK phosphorylation were observed after a single 30-s all-out sprint (Wingate test) in the vastus lateralis.