Establishing reference curves for vital tissue perfusion using quantitative near-infrared fluorescence imaging with indocyanine green

• DOI: 10.1007/s00423-024-03589-1
• Publication Date: 2025-01-08T01:34:28Z
• Authors: Floris P. Tange; Roderick C. Peul; Pim van den Hoven; Stefan Koning; Mo W. Kruiswijk; Robin A. Faber; Pieter S. Verduijn; Carla S. P. van R...; Hidde A. Galema; Denise E. Hilling; Sam P. J. van Dijk; Tessa M. van Ginh...; Stijn Keereweer; Marc A. M. Mureau; Eline A. Feitsma; Milou E. Noltes; Schelto Kruijff; Caroline Driessen; Michael P. Achiam; Abbey Schepers; Jan van Schaik; J. Sven D. Mieog; Alexander L. Vahr...; Jaap F. Hamming; Joost R. van der ...
• Keywords: Indocyanine Green

Abstract Purpose Assessment of tissue perfusion using near-infrared fluorescence (NIR) with indocyanine green (ICG) is gaining popularity, however reliable and objective interpretation remains a challenge. Therefore, this study aimed to establish reference curves for vital across target tissues imaging modality. Methods Data from five prospective cohorts conducted in three Dutch academic medical centres between December 2018 June 2023 was included. Quantitative analysis time-intensity performed ten tissues, including the colon, ileum, gastric conduit, deep inferior epigastric artery perforator (DIEP) flap, skin foot, trachea, sternocleidomastoid muscle (SCM), carotid artery, parathyroid gland, neck. Results A total 178 patients were included study, representing 303 tissues. Three different patterns identified based on subjective assessment. Seven out showed curve rapid inflow (median time-to-max (tmax): 13.0–17.8 s, median maximum-normalized-slope (slope norm): 10.6–12.6%/sec), short outflow area-under-the-curve tmax + 60 s (AUC60): 65.0–85.1%) followed by gradual/absent outflow. Secondly, DIEP flap SCM longer tmax: 24.0, 22.0 slope norm: 9.3, 9.7%/sec respectively) reduced AUC60: 89.1, 89.0% respectively). Thirdly, foot slow 141.1 norm 2.1%/sec) without Conclusion This demonstrates multiple ICG NIR imaging, providing critical step towards clinical implementation technique.