Tie-dyed1 and Sucrose export defective1 act independently to promote carbohydrate export from maize leaves

• DOI: 10.1007/s00425-007-0636-6
• Publication Date: 2007-10-08T08:50:53Z
• Authors: Yi Ma; R. Frank Baker; Maria Magallanes-...; Dean DellaPenna; David M. Braun
• Keywords: Chlorophyll; 0301 basic medicine; 2. Zero hunger; Sucrose; Tocopherols; Plant Science; Zea mays; Carbon; Plant Leaves; 03 medical and health sciences; Phenotype; Microscopy, Electron, Transmission; Microscopy, Fluorescence; Mutation; Genetics; Carbohydrate Metabolism; Original Article; Metabolic Networks and Pathways; Plant Proteins

tie-dyed1 (tdy1) and sucrose export defective1 (sxd1) are recessive maize (Zea mays) mutants with nonclonal chlorotic leaf sectors that hyperaccumulate starch soluble sugars. In addition, both display similar growth-related defects such as reduced plant height inflorescence development due to the retention of carbohydrates in leaves. As tdy1 sxd1 only variegated known accumulate any plant, we investigated whether Tdy1 Sxd1 function same pathway. Using aniline blue staining for callose transmission electron microscopy inspect plasmodesmatal ultrastructure, determined does not have physical blockage or alteration along symplastic transport pathway found mutants. To test two genes genetic pathway, constructed F(2) families segregating mutations. Double mutant plants showed an additive interaction growth related phenotypes sugar accumulation, expressed variegation pattern single indicating act separate pathways. Although lack tocopherols, wild-type tocopherol levels, biochemical Sxd1. From these other data conclude independently promote carbon from Our cytological studies implicate functioning veins, a model discussing possible functions TDY1 is presented.