Occurrence of brassinosteroids and influence of 24-epibrassinolide with brassinazole on their content in the leaves and roots of Hordeum vulgare L. cv. Golden Promise

• DOI: 10.1007/s00425-018-03081-3
• Publication Date: 2018-12-29T03:55:41Z
• Authors: Andrzej Bajguz; Wacław Orczyk; Agnieszka Gołębie...; Magdalena Chmur; Alicja Piotrowska...
• Keywords: Brassinolide; Brassinosteroid; Etiolation

24-epibrassinolide overcame the inhibitory effect of brassinazole on barley growth and content brassinosteroids. The present work demonstrates occurrence mainly castasterone, brassinolide cathasterone lower amounts 24-epibrassinolide, 24-epicastasterone, 28-homobrassinolide, typhasterol, 6-deoxocastasterone 6-deoxotyphasterol in 14-day-old de-etiolated (Hordeum vulgare L. cv. Golden Promise). We also investigated endogenous level brassinosteroids (BRs) seedlings treated with (EBL) and/or (Brz). To our knowledge, this is first report related to BRs application EBL Brz terms barley. as a specific inhibitor BR biosynthetic reactions decreased leaves. Application showed weak promotive Brz-treated seedlings. inhibited seedlings; however, addition inhibition. applied alone at 0.01-1 µM increased leaves but 10 lowered content. In opposition leaves, concentration range from 0.1 1 did not significantly affect roots. However, caused decrease, treatment concentrations Brz.