Evaluation of the protective role of exogenous growth regulators against Ni toxicity in woody shrub Daphne jasminea

• DOI: 10.1007/s00425-018-2979-6
• Publication Date: 2018-08-16T13:05:25Z
• Authors: Alina Wiszniewska; Ewa Muszyńska; Ewa Hanus-Fajerska; Kinga Dziurka; Michał Dziurka
• Keywords: Gibberellic acid; Brassinolide; Jasmonic acid

The results provide a significant verification of the activity exogenously applied phytohormones: gibberellic acid, jasmonic and brassinolide in modulation plant's response to nickel treatment. study investigated accumulation its toxicity Daphne jasminea shoots cultured vitro with or without exogenous supplementation acid (GA3), (JA), (BL). aim was verify modulatory effect plant growth regulators (PGRs) on reaction Ni excess. combined action PGRs evaluated at anatomical, ultrastructural, biochemical levels. Nickel manifested decreased biomass accretion tolerance index (83-53.6%), attributed enhanced synthesis inhibitors, mainly abscisic acid. As defence reaction, endogenous gibberellins accumulated. Exogenous GA3 ameliorated stress, inducing proliferation rate. presence peroxisomal reactions that stimulated JA. In contrast, application BL caused accumulation. Plants suffered from pronounced stress due massive oxidation. strategy plants subjected involved cell wall rearrangements. JA active auxins salicylic contributing mitotic within explants. However, disturbed integrity chloroplasts lamellar compartments. Our revealed an may either enhance increase metal D. jasminea. Particularly culture, where explants are external phytohormonal stimuli, effects supplemental substantially affect development. nickel.