One-step nucleic acid amplification—a molecular method for the detection of lymph node metastases in breast cancer patients; results of the German study group

• DOI: 10.1007/s00428-008-0703-9
• Publication Date: 2008-12-19T10:27:21Z
• Authors: Christian Schem; Nicolai Maass; Dirk O. Bauerschlag; Martin H. Carstensen; Thomas Löning; Christian Roder; Olivera Batic; Walter Jonat; Katharina Tiemann
• Keywords: Concordance; Axillary lymph nodes; Histology

Sentinel lymph node (SN) biopsy is part of the staging procedure in breast cancer patients. In this study, we compared an intraoperative tool named one-step nucleic acid amplification (OSNA) to our routine histological investigation. OSNA consists a short homogenization step followed by cytokeratin (CK) 19 mRNA directly from lysate. To evaluate performance comparison histology, analysis 343 axillary nodes (ALN) 93 patients was performed with both methods. Discordant samples were subjected other If these tests supported results, excluded study. The concordance rate 91.8%, sensitivity 98.1%, and specificity 90.8% before 95.5%, 100%, 95.6%, respectively, after discordant case Our results show that excellent method for detection metastases can be applied as diagnostic approach.