Programmed death-ligand 1 (PD-L1) expression in cervical intraepithelial neoplasia and cervical squamous cell carcinoma of HIV-infected and non-infected patients

• DOI: 10.1007/s00428-023-03580-z
• Publication Date: 2023-06-21T05:03:31Z
• Authors: Maria José Brito; Pedro Sequeira; Ana Quintas; Iryna Silva; Fernanda Silva; Catarina Martins; Ana Félix
• Keywords: Squamous intraepithelial lesion; clone (Java method)

Abstract Programmed death-ligand 1 (PD-L1) is overexpressed in cervical carcinoma, hindering tumor destruction. The aim of this study was to assess PD-L1 expression by immunohistochemistry squamous cell carcinoma (SCC) and intraepithelial lesions (SILs) from human immunodeficiency virus–positive (HIV+) virus-negative (HIV-) patients. A total 166 SCC SIL samples HIV+ HIV- patients were included analyzed for through proportion score (TPS), results stratified five TPS groups using SP263 antibody and, combined positive (CPS) 22C3 antibody. In cohort (SP263 clone), all negative lesion or malignancy (NILM), low-grade (LSILs) scored < 1; 87.5% high-grade (HSILs) adjacent SCC, 19% HSILs non-adjacent 69% SCCs ≥ (15.4% 5). patients, NILM, LSILs, two 1. SCC: 88.2% 5.9% 5. 2 clones), 16.7% with both clones, CPS (22C3) 5 (≥ 50%) (SP263), showing no significant differences positivity between clones. These indicate that a relatively low percentage (16.7%; patients) express (TPS 50% > 1), which may be due some being archival material, sample characteristics, use different methodologies, highlighting the need standardization assessment cervix. fact SILs suggests potential additional applications immunotherapy disease.