The multispecific thyroid hormone transporter OATP1C1 mediates cell-specific sulforhodamine 101-labeling of hippocampal astrocytes

• DOI: 10.1007/s00429-013-0645-0
• Publication Date: 2013-10-15T10:07:56Z
• Authors: Christian Schnell; Ali Shahmoradi; Sven P. Wichert; Steffen Mayerl; Yohannes Hagos; Heike Heuer; Moritz J. Rossner; Swen Hülsmann
• Keywords: Male; Histology; Organic Cation Transport Proteins; Neuroscience(all); Green Fluorescent Proteins; Mice, Transgenic; In Vitro Techniques; Hippocampus; Electrolytes; Mice; 03 medical and health sciences; Animals; Coloring Agents; Cerebral Cortex; 0303 health sciences; Cell-specific markers ; Transcriptome analysis ; Astrocyte; Rhodamines; Computational Biology; 3. Good health; Optogenetics; Animals, Newborn; Astrocytes; Original Article; Female; Anatomy

Sulforhodamine 101 (SR101) is widely used for astrocyte identification, though the labeling mechanism remains unknown and the efficacy of labeling in different brain regions is heterogeneous. By combining region-specific isolation of astrocytes followed by transcriptome analysis, two-photon excitation microscopy, and mouse genetics, we identified the thyroid hormone transporter OATP1C1 as the SR101-uptake transporter in hippocampus and cortex.