GABA–glutamate supramammillary neurons control theta and gamma oscillations in the dentate gyrus during paradoxical (REM) sleep

• DOI: 10.1007/s00429-020-02146-y
• Publication Date: 2020-09-24T06:02:54Z
• Authors: Francesca Billwiller; Laura Castillo; Heba Elseedy; Anton Ivanovich I...; Jennyfer Scapula; Antoine Ghestem; Julien Carponcy; Paul Antoine Libo...; Hélène Bras; Nabila ElSayed Ab...; Esther Krook-Magn...; Ivan Soltesz; Christophe Bernard; Pierre-Hervé Luppi; Monique Esclapez
• Keywords: Neurochemical; Granule cell

Abstract Several studies suggest that neurons from the lateral region of SuM (SuML) innervating dorsal dentate gyrus (DG) display a dual GABAergic and glutamatergic transmission are specifically activated during paradoxical (REM) sleep (PS). The objective present study is to characterize anatomical, neurochemical electrophysiological properties SuML-DG projection determine how they control DG oscillations neuronal activation PS other vigilance states. For this purpose, we combine structural connectivity techniques using neurotropic viral vectors (rabies virus, AAV), anatomy (immunohistochemistry, in situ hybridization) imaging (light, electron confocal microscopy) with vitro (patch clamp) vivo (LFP, EEG) optogenetic recordings performed transgenic VGLUT2-cre male mice. At cellular level, show co-release GABA glutamate on granule cells increase activity subset cells. network pathway increases theta power frequency as well gamma waking DG. behavioral does not change animal behavior PS, induces awakening slow wave motor waking. These results capable supporting observed plays an important modulatory role state.