Association of clinicopathological features with UbcH10 expression in colorectal cancer

• DOI: 10.1007/s00432-009-0672-7
• Publication Date: 2009-09-24T11:14:51Z
• Authors: Shimin Chen; Yingjian Chen; Chengjin Hu; Hongbiao Jing; Yongcheng Cao; Xianxi Liu
• Keywords: Adult; Aged, 80 and over; Male; 0303 health sciences; Carcinoma; Cell Differentiation; Middle Aged; Prognosis; Tumor Burden; Up-Regulation; 3. Good health; Gene Expression Regulation, Neoplastic; 03 medical and health sciences; Cell Transformation, Neoplastic; Ubiquitin-Conjugating Enzymes; Disease Progression; Humans; Female; RNA, Small Interfering; Colorectal Neoplasms; HT29 Cells; Aged

UbcH10 is the cancer-related E2 ubiquitin-conjugating enzyme, and its overexpression has been demonstrated in a variety of malignancies. The aim of this study is to investigate the association of UbcH10 gene expression with the carcinogenesis and tumor progression of colorectal cancer.The expression levels of UbcH10 in human malignant colorectal carcinoma tissues and their adjacent normal tissues were examined using real-time quantitative RT-PCR and immunohistochemical analysis. The correlations of UbcH10 expression to the clinicalpathologic characteristics of the colorectal cancer were analyzed. Cell proliferation and Matrigel invasion assays were performed in HT-29 cells transfected with UbcH10 expression plasmid pcDNA3.1-UbcH10, UbcH10 RNA interference vector pUbcH10-RNAi as well as their control vectors.Our study demonstrated that the expression of UbcH10 in colorectal carcinoma tissues was significantly higher than that in non-cancerous tissues (P < 0.01), and the UbcH10 overexpression was related to the degree of tumor differentiation and lymph node metastasis of colorectal cancer patients (P < 0.05). In vitro, the overexpression of UbcH10 promoted cell proliferation and tumor invasiveness, but the downregulation of UbcH10 expression significantly reduced the growth rate and the invasiveness activity of tumor cell line.Our study suggests that the overexpression of UbcH10 gene plays a critical role in the carcinogenesis and tumor progression of colorectal cancer. It may be a new marker in diagnosis and prognosis of colorectal cancer, and the inhibition of UbcH10 may be a therapeutic potential for the treatment of colorectal cancer.