Expellency, anti-feeding and speed of kill of a dinotefuran-permethrin-pyriproxyfen spot-on (Vectra®3D) in dogs weekly challenged with adult fleas (Ctenocephalides felis) for 1 month—comparison to a spinosad tablet (Comfortis®)

• DOI: 10.1007/s00436-015-4470-7
• Publication Date: 2015-04-13T19:31:12Z
• Authors: Marie Varloud; Josephus J Fourie; Byron L Blagburn; Audrey Deflandre
• Keywords: Pyriproxyfen; Ctenocephalides; Piperonyl butoxide

This study was designed to compare the efficacy of two ectoparasiticides against adult fleas on dogs: a topical (DPP, dinotefuran-permethrin-pyriproxyfen) and systemic (S, spinosad). Dogs (n = 48; 10.21-22.86 kg BW) were allocated six groups eight dogs each (C1, C4, DPP1, DPP4, S1, S4). in treated administered (3.6 mL DPP) or tablet (665 1040 mg S) day 0. Infestations with 100 unfed (Ctenocephalides felis) occurred days -6, -1, 2, 7, 14, 21 28. An additional untreated group (QC, n 6) involved evaluate flea-anti-feeding efficacy. These infested once 150 prior combing at least 50 live from dog 5 10 min after infestation. In group, dislodged dead moribund collected 5, 10, 15 60 (DPP1, S1) 30 240 (DPP4, S4) post-treatment subsequent flea infestations pans placed underneath cages. Fleas counted removed by 1 4 h (C4, infestations. Quantitative PCR analysis canine cytochrome b gene conducted control (QC) The number copies used as marker blood volume ingested fleas. Dislodgeability insecticidal calculated using arithmetic means. A rapid onset killing observed for DPP 12.7 % being average soon exhibited significantly higher sustained speed kill than S. 86 ± 8.8 95.3 2.1 DPP, whereas it only 33.7 19.9 57.6 18.6 S respectively weekly reinfestations. combination inhibited feeding (89 reduction) up mortality 1-month post-treatment.