Occurrence and molecular characterization of Giardia duodenalis in lambs in Djelfa, the central steppe of Algeria

• DOI: 10.1007/s00436-020-06808-y
• Publication Date: 2020-07-14T02:02:21Z
• Authors: Soumaya Benhassine; Djamel Baroudi; Ahcene Hakem; Myriam Thomas; AbdElkarim Laatamna; Salem Belkessa; Yaoyu Feng; Dawn Roellig; Xiaohua Chen; Karim Tarik Adjou; Lihua Xiao
• Keywords: Giardiasis; 0301 basic medicine; Genotype; Epidemiology; Protozoan Proteins; 610; Cattle Diseases; Sheep Diseases; Zoonosis; Feces; 03 medical and health sciences; MESH: Giardia lamblia; Glutamate Dehydrogenase; Prevalence; Animals; [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology; Phylogeny; 2. Zero hunger; Sheep; MESH: Giardiasis; MESH: Sheep Diseases; 600; Lambs; [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology; Algeria; [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie; Cattle; Giardia lamblia; Giardia duodenalis; Triose-Phosphate Isomerase

Little is known of the prevalence and genetic identity of Giardia duodenalis in sheep in Algeria. The present study aimed at characterizing G. duodenalis in lambs up to 6 months of age in Djelfa, Algeria. A total of 346 fecal specimens were collected from 28 farms and screened for G. duodenalis cysts by zinc sulfate flotation microscopy, and positive specimens were confirmed using a direct immunofluorescence assay. Microscopy-positive specimens were analyzed by PCR and sequence analysis of the triosephosphate isomerase and glutamate dehydrogenase genes to determine G. duodenalis assemblages. Coprological examination indicated that the overall infection rate was 7.0% (24/346). Lambs under 3 months of age had higher infection rate (18/197, 9.0%) than older (6/149, 4.0%) animals, and animals with diarrhea (7/44, 16.0%) had higher infection rate than animals without diarrhea (17/302, 5.6%). PCR sequence analyses of the 15 G. duodenalis isolates revealed the presence of assemblages A in 6 isolates, assemblage E in 7 isolates, and both in 2 isolates. Assemblage A was only found in pre-weaned lambs with diarrhea, while assemblage E was mostly found in post-weaned lambs without diarrhea. The assemblage E isolates from sheep were genetically related to those from cattle in Algeria, while assemblage A isolates were from a well-known subtype prevalent in humans. Data generated from the study improve our understanding of the transmission of G. duodenalis in Algeria.