A SYBR green I real-time polymerase chain reaction (PCR) assay for detection and quantification of Trichomonas gallinae

• DOI: 10.1007/s00436-020-06887-x
• Publication Date: 2020-09-22T05:02:51Z
• Authors: Zaida Rentería-Solís; Tran Nguyen-Ho-Bao; Shahinaz Taha; Arwid Daugschies
• Keywords: 0301 basic medicine; Protozoology - Short Communication; Reproducibility of Results; Trichomonas Infections; Diamines; SYBR green, ; Organic Chemicals/metabolism [MeSH] ; Trichomonas Infections/parasitology [MeSH] ; Trichomonas Infections/diagnosis [MeSH] ; Birds/parasitology [MeSH] ; RNA, Ribosomal, 18S/genetics [MeSH] ; Host-Pathogen Interactions [MeSH] ; Animals [MeSH] ; Flagellates ; Phylogeny [MeSH] ; Real-time PCR, ; Sensitivity and Specificity [MeSH] ; Reproducibility of Results [MeSH] ; Trichomoniasis, ; Parasitology/methods [MeSH] ; Protozoology - Short Communication ; Trichomonas/genetics [MeSH] ; Real-Time Polymerase Chain Reaction [MeSH]; Real-Time Polymerase Chain Reaction; Sensitivity and Specificity; 3. Good health; Birds; 03 medical and health sciences; Host-Pathogen Interactions; Quinolines; RNA, Ribosomal, 18S; Trichomonas; Animals; Parasitology; Benzothiazoles; Organic Chemicals; Phylogeny

AbstractTrichomonas gallinae are parasitic flagellates of importance in wild and domestic birds. The parasite is worldwide distributed, and Columbine birds are its main host. Current research focuses mostly on epidemiological and phylogenetic studies. However, there is still a lack of knowledge regarding parasite-host interaction or therapy development. Real-time PCR is a useful tool for diagnostic and quantification of gene copies in a determined sample. By amplification of a 113-bp region of the 18S small subunit ribosomal RNA gene, a SYBR green-based real-time PCR assay was developed. A standard curve was prepared for quantification analysis. Assay efficiency, linearity, and dissociation analysis were successfully performed. Specificity, sensibility, and reproducibility analysis were tested. This assay could be a useful tool not only for diagnostic purposes but also for future in vivo and in vitro T. gallinae studies.