Circular RNA screening identifies circMYLK4 as a regulator of fast/slow myofibers in porcine skeletal muscles

• DOI: 10.1007/s00438-021-01835-5
• Publication Date: 2021-11-16T19:04:01Z
• Authors: Haigang Cao; Jieming Liu; Tianning Du; Yihao Liu; Xiaoyu Zhang; Yuan Guo; Jie Wang; Xiaomin Zhou; Xiao Li; Gongshe Yang; Xin’e Shi
• Keywords: Circular RNA; Developmental Biology

The type of myofiber is related to the quality meat. slow oxidized helps increase tenderness and juiciness muscle. Numerous studies have shown that circRNA plays a key role in skeletal muscle development. However, porcine types unclear. In this study, we performed high-throughput RNA sequencing study differential expression longissimus dorsi soleus A total 40,757 circRNAs were identified, which 181 significantly different. Interestingly, some involved metabolism pathways, AMPK, FoxO, PI3K-Akt signaling pathways. Besides, focused on novel circRNA-circMYLK4. By injecting circMYLK4-AAV into piglets, found circMYLK4 increased mRNA protein levels marker genes. summary, our laid an essential foundation for further research conversion higher meat quality.