Covalent immobilization of β-galactosidase using a novel carrier alginate/tea waste: statistical optimization of beads modification and reusability

• DOI: 10.1007/s00449-023-02959-1
• Publication Date: 2024-01-10T05:02:05Z
• Authors: Mohamed A. A. Abd...; Mohamed E. Hassan
• Keywords: Reusability

Abstract β-galactosidase has been immobilized onto novel alginate/tea waste gel beads (Alg/TW) via covalent binding. Alg/TW were subjected to chemical modification through amination with polyethyleneimine (PEI) followed by activation glutaraldehyde (GA). Chemical parameters including PEI concentration, pH, and GA concentration statistically optimized using Response Surface methodology (RSM) based on Box–Behnken Design (BBD). Analysis of variance (ANOVA) results confirmed the great significance model that had F value 37.26 P < 0.05. Furthermore, R 2 (0.9882), Adjusted (0.9617), predicted (0.8130) referred high correlation between experimental values, demonstrating fitness model. In addition, coefficient variation (CV) was 2.90 pointed accuracy experiments. The highest immobilization yield (IY) (75.1%) given under conditions (4%), pH (9.5), (2.5%). characterized FT-IR, TGA, SEM techniques at each step process. Moreover, revealed a very good reusability as it could be reused for 15 20 consecutive cycles keeping 99.7 72.1% its initial activity, respectively. conclusion, environmental (tea waste) can used in modern technological industries such food pharmaceutical industry.