The meiotic topoisomerase VI B subunit (MTOPVIB) is essential for meiotic DNA double-strand break formation in barley (Hordeum vulgare L.)

• DOI: 10.1007/s00497-022-00444-5
• Publication Date: 2022-06-29T04:03:55Z
• Authors: Stefan Steckenborn; Maria Cuacos; Mohammad A. Ayoub; Chao Feng; Veit Schubert; Iris Hoffie; Götz Hensel; Jochen Kumlehn; Stefan Heckmann
• Keywords: 0301 basic medicine; 2. Zero hunger; Meiosis; 03 medical and health sciences; DNA Topoisomerases, Type II; DNA Repair; Archaeal Proteins; Original Article; Hordeum; DNA; Meiosis ; Meiotic recombination ; Barley ; MTOPVIB ; Meiotic spindle ; Meiotic DSB

Abstract Key message In barley (Hordeum vulgare), MTOPVIB is critical for meiotic DSB and accompanied SC and CO formation while dispensable for meiotic bipolar spindle formation.

Abstract Homologous recombination during meiosis assures genetic variation in offspring. Programmed meiotic DNA double-strand breaks (DSBs) are repaired as crossover (CO) or non-crossover (NCO) during meiotic recombination. The meiotic topoisomerase VI (TopoVI) B subunit (MTOPVIB) plays an essential role in meiotic DSB formation critical for CO-recombination. More recently MTOPVIB has been also shown to play a role in meiotic bipolar spindle formation in rice and maize. Here, we describe a meiotic DSB-defective mutant in barley (Hordeum vulgare L.). CRISPR-associated 9 (Cas9) endonuclease-generated mtopVIB plants show complete sterility due to the absence of meiotic DSB, synaptonemal complex (SC), and CO formation leading to the occurrence of univalents and their unbalanced segregation into aneuploid gametes. In HvmtopVIB plants, we also frequently found the bi-orientation of sister kinetochores in univalents during metaphase I and the precocious separation of sister chromatids during anaphase I. Moreover, the near absence of polyads after meiosis II, suggests that despite being critical for meiotic DSB formation in barley, MTOPVIB seems not to be strictly required for meiotic bipolar spindle formation.