Impact of sirtuin-1 expression on H3K56 acetylation and oxidative stress: a double-blind randomized controlled trial with resveratrol supplementation

• DOI: 10.1007/s00592-017-1097-4
• Publication Date: 2018-01-12T06:51:52Z
• Authors: Simona Bo; Gabriele Togliatto; Roberto Gambino; Valentina Ponzo; Giusy Lombardo; Rosalba Rosato; Maurizio Cassader; Maria Felice Brizzi
• Keywords: Sirtuin 1

Abstract Aims Sirtuin-1 (SIRT-1) down-regulation in type 2 diabetes mellitus (T2DM) has been associated with epigenetic markers of oxidative stress. We herein aim to evaluate whether an increase SIRT-1 expression affects histone 3 acetylation at the 56 lysine residue (H3K56ac) T2DM patients randomly selected receive either resveratrol (40 mg or 500 mg) a placebo for 6 months. The primary outcome is changes H3K56ac level by variation and secondary evidence association between level, antioxidant (TAS), metabolic variables. Methods results At baseline, peripheral blood mononuclear cell values among tertiles did not differ. trial end, levels were significantly higher receiving resveratrol. follow-up, divided into delta (trial end minus baseline) value. Significant reductions body fat percentage found highest tertile as increased TAS levels. A multiple logistic regression model showed that was inversely variations (OR = 0.66; 95% CI 0.44–0.99), 1.01; 1.00–1.02), 0.75; 0.58–0.96). Conclusions provide new knowledge on T2DM. These data suggest boosting expression/activation may impact redox homeostasis these patients. ClinicalTrials.gov Identifier NCT02244879.